Cranberry oil is known for nutritional benefits, and this work is aimed at studying its soothing properties and potential as an intimate care ingredient. The antioxidant, anti-inflammatory, and anti-irritation properties of cranberry oil were evaluated on epithelial cells and tissues, including the vaginal epithelium. The impact of the oil on vaginal microbiota was assessed in vitro. Cranberry oil reduced oxidative stress in keratinocytes (ROS -43%) and lowered inflammation by lessening the release of cytokines IL-8 (-33%) and TNF- alpha (-32%). Irritation induced by sodium dodecyl sulfate (SDS) in skin explants was lowered by 24%. Cranberry oil and fruit extract acted synergistically on inflammation, decreasing TNF- alpha release by 75% (vs. -34% and -16%, respectively). Cranberry oil reduced inflammation on EpiVaginalTM tissue, decreasing IL-6 by 36%. The minimum inhibitory concentration (MIC) of cranberry oil on the pathogenic vaginal microorganisms C. albicans and G. vaginalis was 0.5% and 0.1%, respectively. The oil promoted the growth of commensal L. jensenii (x79 at 0.1%) and favored a high proportion of lactic acid bacteria when co-cultured with C. albicans. Cranberry oil has antioxidant, anti-inflammatory, and soothing properties on skin. Anti-inflammatory activity was confirmed on vaginal epithelium, and initial in vitro evidence indicates that the oil can balance vaginal flora to prevent dysbiosis.